Aptamer-modified micellar electrokinetic chromatography for the enantioseparation of nucleotides.

@article{Ruta2009AptamermodifiedME,
  title={Aptamer-modified micellar electrokinetic chromatography for the enantioseparation of nucleotides.},
  author={Jos{\'e}phine Ruta and Sandrine Perrier and Corinne Ravelet and B{\'e}atrice Roy and Christian P{\'e}rigaud and Eric Peyrin},
  journal={Analytical chemistry},
  year={2009},
  volume={81 3},
  pages={1169-76}
}
In this paper, a new aptamer-based capillary electrophoresis (CE) method, which was able to separate the enantiomers of an anionic target (adenosine monophosphate, AMP) displaying the same electrophoretic mobility as that of the oligonucleotidic chiral selector, is reported. The design of the aptamer-modified micellar electrokinetic chromatography (MEKC) mode consisted of nonionic micelles which acted as a pseudostationary phase and a hydrophobic cholesteryl group-tagged aptamer (Chol-Apt… CONTINUE READING