Aptamer/thrombin/aptamer-AuNPs sandwich enhanced surface plasmon resonance sensor for the detection of subnanomolar thrombin.

@article{Bai2013AptamerthrombinaptamerAuNPsSE,
  title={Aptamer/thrombin/aptamer-AuNPs sandwich enhanced surface plasmon resonance sensor for the detection of subnanomolar thrombin.},
  author={Yunfeng Bai and Feng Feng and Lu Zhao and Chunyu Wang and Haiyan Wang and Maozhong Tian and Jun Qin and Yali Duan and Xiaoxiao He},
  journal={Biosensors & bioelectronics},
  year={2013},
  volume={47},
  pages={265-70}
}
A sensitive and selective aptamer/thrombin/aptamer-AuNPs sandwich enhanced surface plasmon resonance (SPR) sensor has been developed for real-time detection of subnanomolar thrombin. In this protocol, one thiol-modified thrombin aptamer (TBA29) was immobilized on gold nanoparticles (AuNPs) via Au-S bonding. The other biotinylated thrombin aptamer (TBA15) was grafted onto streptavidin pretreated SPR gold film through biotin-streptavidin recognition. The presence of thrombin would then induce the… CONTINUE READING

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