Approaching real-time molecular diagnostics: single-pair fluorescence resonance energy transfer (spFRET) detection for the analysis of low abundant point mutations in K-ras oncogenes.

@article{Wabuyele2003ApproachingRM,
  title={Approaching real-time molecular diagnostics: single-pair fluorescence resonance energy transfer (spFRET) detection for the analysis of low abundant point mutations in K-ras oncogenes.},
  author={Musundi B. Wabuyele and Hannah D Farquar and Wieslaw J. Stryjewski and Robert P. Hammer and Steven Allan Soper and Yu-Wei Cheng and Francis Barany},
  journal={Journal of the American Chemical Society},
  year={2003},
  volume={125 23},
  pages={6937-45}
}
The aim of this study was to develop new strategies for analyzing molecular signatures of disease states approaching real-time using single pair fluorescence resonance energy transfer (spFRET) to rapidly detect point mutations in unamplified genomic DNA. In addition, the detection process was required to discriminate between normal and mutant (minority) DNAs in heterogeneous populations. The discrimination was carried out using allele-specific primers, which flanked the point mutation in the… CONTINUE READING

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