Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation.

Abstract

MicroRNAs (miRNAs) are crucial components of the molecular networks regulating differentiation and responses of T lymphocytes in health and disease. It is therefore essential to rely on robust methods of qualitative and quantitative investigation of miRNA expression in T cell subsets, and during T cell activation and differentiation. Here, we focus on different methods for miRNA analysis, including Northern blots, quantitative RT-PCR, and next-generation sequencing, and we discuss advantages and disadvantages of each method. While we mainly focus on the study of miRNA expression in human T lymphocytes, these methods can also be applied to other species and/or cell types.

Cite this paper

@article{Monticelli2017ApproachesTD, title={Approaches to Detect microRNA Expression in T Cell Subsets and T Cell Differentiation.}, author={Silvia Monticelli and Tarmo {\"Aij{\"{o} and Sara Trifari}, journal={Methods in molecular biology}, year={2017}, volume={1514}, pages={153-172} }