Applying the increase in rate constants of cooperative proteolysis to the determination of transition curves of protein denaturation.

Abstract

The transition curves of soybean glycinin egg lysozyme, bovine serum albumin (BSA) denaturation under the action of increasing urea concentration were monitored by determination of the increase in rate constants of cooperative enzymatic proteolysis. The results were compared with those obtained using intrinsic fluorescence and the number of accessible… (More)

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