Applying pairwise combinations of amino acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides.

Abstract

Iterative saturation mutagenesis and combinatorial active site saturation focused on vicinal amino acids were used to alter the acceptor specificity of amylosucrase from Neisseria polysaccharea , a sucrose-utilizing α-transglucosidase, and sort out improved variants. From the screening of three semirational sublibraries accounting in total for 20,000… (More)
DOI: 10.1021/ja306845b

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Cite this paper

@article{Champion2012ApplyingPC, title={Applying pairwise combinations of amino acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides.}, author={Elise Champion and Fr{\'e}d{\'e}ric Gu{\'e}rin and Claire Moulis and Sophie Barbe and Thu Hoai Tran and Sandrine Morel and Karine Descroix and Pierre Monsan and Lionel Mourey and Laurence A. Mulard and Samuel Tranier and Magali Remaud-Sim{\'e}on and Isabelle Andr{\'e}}, journal={Journal of the American Chemical Society}, year={2012}, volume={134 45}, pages={18677-88} }