Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells

@article{Schrder2011ApplyingLD,
  title={Applying label-free dynamic mass redistribution technology to frame signaling of G protein–coupled receptors noninvasively in living cells},
  author={Ralf Schr{\"o}der and Johannes P Schmidt and Stefanie Bl{\"a}ttermann and Lucas Peters and Nicole Janssen and Manuel Grundmann and Wiebke K. Seemann and Dorina Kaufel and Nicole Merten and Christel Drewke and Jes{\'u}s Gomeza and Graeme Milligan and K. -H. Mohr and Evi Kostenis},
  journal={Nature Protocols},
  year={2011},
  volume={6},
  pages={1748-1760}
}
Label-free dynamic mass redistribution (DMR) is a cutting-edge assay technology that enables real-time detection of integrated cellular responses in living cells. It relies on detection of refractive index alterations on biosensor-coated microplates that originate from stimulus-induced changes in the total biomass proximal to the sensor surface. Here we describe a detailed protocol to apply DMR technology to frame functional behavior of G protein–coupled receptors that are traditionally… CONTINUE READING