Applying CRISPR/Cas for genome engineering in plants: the best is yet to come.

  title={Applying CRISPR/Cas for genome engineering in plants: the best is yet to come.},
  author={Holger Puchta},
  journal={Current opinion in plant biology},
Less than 5 years ago the CRISPR/Cas nuclease was first introduced into eukaryotes, shortly becoming the most efficient and widely used tool for genome engineering. For plants, efforts were centred on obtaining heritable changes in most transformable crop species by inducing mutations into open reading frames of interest, via non-homologous end joining. Now it is important to take the next steps and further develop the technology to reach its full potential. For breeding, besides using DNA-free… CONTINUE READING
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