Applications of single-wavelength anomalous dispersion at high and atomic resolution.

@article{Brodersen2000ApplicationsOS,
  title={Applications of single-wavelength anomalous dispersion at high and atomic resolution.},
  author={Ditlev Egeskov Brodersen and Eric de La Fortelle and Clemens Vonrhein and Gerard Bricogne and Jens Nyborg and Morten Kjeldgaard},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={2000},
  volume={56 Pt 4},
  pages={431-41}
}
Two examples of the application of single-wavelength anomalous dispersion (SAD) in macromolecular structure determination are described, both using the statistical phasing program SHARP. For the holmium-substituted calcium-binding protein psoriasin (22.7 kDa), a set of accurate phases has been obtained to a resolution of 1.05 A without recourse to an atomic model of the molecule. The accuracy of the phases resulted in an electron-density map of a quality comparable to sigma(A)-weighted 2mF(o… CONTINUE READING

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