Applications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples

  title={Applications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples},
  author={Tong Zhang and Herbert Han-ping Fang},
  journal={Applied Microbiology and Biotechnology},
  • Tong Zhang, H. Fang
  • Published 10 February 2006
  • Biology
  • Applied Microbiology and Biotechnology
Due to the advanced development of fluorogenic chemistry, quantitative real-time polymerase chain reaction (qRT-PCR) has become an emerging technique for the detection and quantification of microorganisms in the environment. Compared with the conventional hybridization- and PCR-based techniques, qRT-PCR not only has better sensitivity and reproducibility, but it is also quicker to perform and has a minimum risk of amplicon carryover contamination. This article reviews the principle of this… 

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