Applications of pressure perturbation calorimetry to study factors contributing to the volume changes upon protein unfolding.

BACKGROUND Pressure perturbation calorimetry (PPC) is a biophysical method that allows direct determination of the volume changes upon conformational transitions in macromolecules. SCOPE OF THIS REVIEW This review provides novel details of the use of PPC to analyze unfolding transitions in proteins. The emphasis is made on the data analysis as well as on… (More)