Applications of phasors to in vitro time-resolved fluorescence measurements.

Abstract

The phasor method of treating fluorescence lifetime data provides a facile and convenient approach to characterize lifetime heterogeneity and to detect the presence of excited state reactions such as solvent relaxation and Förster resonance energy transfer. The method uses a plot of M sin(Φ) versus M cos(Φ), where M is the modulation ratio and Φ is the… (More)
DOI: 10.1016/j.ab.2010.11.010

Topics

Cite this paper

@article{tefl2011ApplicationsOP, title={Applications of phasors to in vitro time-resolved fluorescence measurements.}, author={Martin {\vS}tefl and Nicholas James and Justin A. Ross and D. Jameson}, journal={Analytical biochemistry}, year={2011}, volume={410 1}, pages={62-9} }