Applications of gene replacement technology to Streptomyces clavuligerus strain development for clavulanic acid production.

@article{Paradkar2001ApplicationsOG,
  title={Applications of gene replacement technology to Streptomyces clavuligerus strain development for clavulanic acid production.},
  author={Ashish S. Paradkar and Roy H. Mosher and Cecilia L Anders and Alison R Griffin and John H. Griffin and Christine A Hughes and Philip Greaves and Barry Barton and Susan E. Jensen},
  journal={Applied and environmental microbiology},
  year={2001},
  volume={67 5},
  pages={2292-7}
}
Cephamycin C production was blocked in wild-type cultures of the clavulanic acid-producing organism Streptomyces clavuligerus by targeted disruption of the gene (lat) encoding lysine epsilon-aminotransferase. Specific production of clavulanic acid increased in the lat mutants derived from the wild-type strain by 2- to 2.5-fold. Similar beneficial effects on clavulanic acid production were noted in previous studies when gene disruption was used to block the production of the non-clavulanic acid… CONTINUE READING

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Probable role of clavaminic acid as the terminal intermediate in the common pathway to clavulanic acid and the antipodal clavam metabolites

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