Applications of chemical tagging approaches in combination with 2DE and mass spectrometry.

Abstract

Chemical modification reactions play an important role in various protocols for mass-spectrometry-based proteome analysis; this applies to both gel-based and gel-free proteomics workflows. In combination with two-dimensional gel electrophoresis (2DE), the addition of "tags" by means of chemical reactions serves several purposes. Potential benefits include… (More)
DOI: 10.1007/978-1-59745-281-6_6

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