Applications of SYPRO orange and SYPRO red protein gel stains.

  title={Applications of SYPRO orange and SYPRO red protein gel stains.},
  author={Thomas H. Steinberg and Richard P. Haugland and Victoria L. Singer},
  journal={Analytical biochemistry},
  volume={239 2},
We have further characterized the sensitivity and specificity of SYPRO Orange protein gel stain and SYPRO Red protein gel stain with native and 2-dimensional polyacrylamide gels and for staining gels prior to Western blot analysis. We found that nucleic acids are not stained by the SYPRO protein gel stains, in contrast to results obtained with commonly used silver staining techniques. Bacterial lipopolysaccharides also stain very weakly with SYPRO dyes in comparison to silver staining. Thus… CONTINUE READING


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