Applications of Loop-Mediated Isothermal DNA Amplification

@article{Fu2011ApplicationsOL,
  title={Applications of Loop-Mediated Isothermal DNA Amplification},
  author={Shijun Fu and Guanggang Qu and Shijin Guo and Linares Uribe Ma and Na Zhang and Songlin Zhang and Sanyang Gao and Zhiqiang Shen},
  journal={Applied Biochemistry and Biotechnology},
  year={2011},
  volume={163},
  pages={845-850}
}
During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation… Expand
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LAMP technology, with the integration of these features, can rightly apply to clinical genetic testing, food and environmental analysis, as well as NAT in different fields. Expand
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Among nasopharyngeal swab specimens from 120 patients with acute respiratory tract infections and 40 healthy individuals, the LAMP results showed 100% agreement with the results of real-time polymerase chain reaction (PCR) assays. Expand
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