Applications of CRISPR technologies in research and beyond

  title={Applications of CRISPR technologies in research and beyond},
  author={Rodolphe Barrangou and Jennifer A. Doudna},
  journal={Nature Biotechnology},
Programmable DNA cleavage using CRISPR–Cas9 enables efficient, site-specific genome engineering in single cells and whole organisms. In the research arena, versatile CRISPR-enabled genome editing has been used in various ways, such as controlling transcription, modifying epigenomes, conducting genome-wide screens and imaging chromosomes. CRISPR systems are already being used to alleviate genetic disorders in animals and are likely to be employed soon in the clinic to treat human diseases of the… 

Development and application of CRISPR/Cas9 technologies in genomic editing.

The application of the CRISPR system over the last 2 years is reviewed, including its development and application in base editing, transcription modulation and epigenetic editing, genomic-scale screening, and cell and embryo therapy.

In vivo genome editing thrives with diversified CRISPR technologies

The recent developments and applications of CRISPR-based technology in generating various animal models are reviewed, and the everlasting impact of this new progress on biomedical research is discussed.

Viral Vectors, Engineered Cells and the CRISPR Revolution.

This chapter seeks to highlight and review important progress in the realm of the editing of human cells using CRISPR-Cas systems, the use of viruses as vectors for gene therapy, and the application of engineered cells to study and treat disease.

Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells.

A detailed protocol discussing the necessary steps for the successful performance of pooled CRISPR-Cas9 screens is provided, providing a powerful tool for functional genomics with manifold applications.

Expansion of the CRISPR/Cas Genome-Sculpting Toolbox: Innovations, Applications and Challenges.

This review provides an illustrative overview of the current research trends using CRISPR/Cas technology and highlights the latest developments in CRISpr/Cas technique includingCRISPR imaging, discovery of novel CRISP systems, and applications in altering the genome, epigenome or RNA in different organisms.

Crispr/Cas9 System - A Revolution in Gene Editing

  • T. Daneva
  • Biology
    Biomedical Journal of Scientific & Technical Research
  • 2018
This technology has opened countless opportunities for easy, inexpensive, and quick editing of human genes and literally has the potential to change the human medicine and health.

CRISPR/Cas9 system: a reliable and facile genome editing tool in modern biology

This review presents a comprehensive knowledge about the mechanism and structure of Cas9-mediated RNA-guided DNA targeting and cleavage, and genome editing via CRISPR-Cas9 technology in various animals which are being used as models in scientific research has been discussed in this review.



Applications of CRISPR–Cas systems in neuroscience

Precise and efficient gene editing using CRISPR–Cas systems has the potential to advance both basic and translational neuroscience research.

Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference

How the history of RNAi can inform today's challenges in CRISPR-Cas9 genome engineering such as efficiency, specificity, high-throughput screening and delivery for in vivo and therapeutic applications is examined.

Cas9 Targeting and the CRISPR Revolution

This work has shown that genome editing technology based on the prokaryotic CRISPR–Cas9 system is completely revolutionizing genome engineering and has already started to supplant incumbent genome editing technologies, such as TALENs and ZFNs.

Inducible in vivo genome editing with CRISPR/Cas9

It is shown that doxycycline-regulated Cas9 induction enables widespread gene disruption in multiple tissues and that limiting the duration of Cas9 expression or using a Cas9D10A (Cas9n) variant can regulate the frequency and size of target gene modifications, respectively.

Efficient genome editing in zebrafish using a CRISPR-Cas system.

It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.