Application of typing by pulsed-field gel electrophoresis to the study of Clostridium difficile in a neonatal intensive care unit.

@article{Kato1994ApplicationOT,
  title={Application of typing by pulsed-field gel electrophoresis to the study of Clostridium difficile in a neonatal intensive care unit.},
  author={Hidehito Kato and Naoki Kato and Kunitomo Watanabe and Kazue Ueno and Hiroshi Ushijima and Shintaro Hashira and Takashi Abe},
  journal={Journal of clinical microbiology},
  year={1994},
  volume={32 9},
  pages={2067-70}
}
Pulsed-field gel electrophoresis (PFGE) analysis of restriction pattern polymorphism was applied to type Clostridium difficile isolated from neonates hospitalized in a neonatal intensive care unit, and the results were compared with those of immunoblot analysis. C. difficile was isolated from fecal specimens of 41 (61%) of 67 neonates during a 5-month investigation. All of these neonates were asymptomatic. Fifty-five C. difficile isolates from 32 patients were analyzed by PFGE after digestion… CONTINUE READING