Application of triphenyltetrazolium chloride in microbial limit test of pharmaceuticals and cosmetics.

@article{Ohara1995ApplicationOT,
  title={Application of triphenyltetrazolium chloride in microbial limit test of pharmaceuticals and cosmetics.},
  author={Mitsuko Taba Ohara and Tomonori Saito},
  journal={Journal of AOAC International},
  year={1995},
  volume={78 6},
  pages={1525-9}
}
An alternative method was developed for counting viable microbial contaminants in pharmaceuticals and cosmetics with insoluble materials and low bioburden. The method uses triphenyltetrazolium chloride (TTC). The inhibitory effect of TTC on microbial growth observed by others was eliminated by adding it as an overlay, after incubation of sample in culture… CONTINUE READING