Application of thin layer chromatography to histamine radioenzymatic assay

@article{Dent1979ApplicationOT,
  title={Application of thin layer chromatography to histamine radioenzymatic assay},
  author={C. E. Dent and F. Nilam and Ian R. Smith},
  journal={Agents and Actions},
  year={1979},
  volume={9},
  pages={34-35}
}
Introduction The enzymatic radiochemical assay for histamine, developed by SNYDER et al. [1] and modified by a number of groups (see, for example, review by BEAVEN [2]) is both sensitive and specific. The procedure involves converting histamine into radiolabelled 3-methylhistamine, catalysed by the enzyme histamine N-methyltransferase with S-adenosylmethionine (SAM), radiolabelled in the methyl group, as methyl group donor. A disadvantage of the assay is that it cannot be used to measure… CONTINUE READING