Application of the zona-free manipulation technique to porcine somatic nuclear transfer.

@article{Booth2001ApplicationOT,
  title={Application of the zona-free manipulation technique to porcine somatic nuclear transfer.},
  author={Paul Booth and Sherylle J Tan and Peter Holm and H Callesen},
  journal={Cloning and stem cells},
  year={2001},
  volume={3 4},
  pages={191-7}
}
The recent demonstration of a successful zona-free manipulation technique for bovine somatic nuclear transfer (NT) that is both simpler and less labor intensive is of considerable benefit to advance the applications of this technology. Here, we describe that this method is also applicable to porcine somatic NT. Porcine cumulus oocyte complexes were matured in TCM-199 medium before sequential removal of the cumulus and zonae. Zona-free oocytes were bisected using a microknife, and the halves… CONTINUE READING

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