Application of the upstream region of a Bacillus endoglucanase gene to high-level expression of foreign genes in Bacillus subtilis.

@article{Sumitomo1995ApplicationOT,
  title={Application of the upstream region of a Bacillus endoglucanase gene to high-level expression of foreign genes in Bacillus subtilis.},
  author={Nobuyuki Sumitomo and Katsuya Ozaki and Jun Hitomi and Shunro Kawaminami and T. Kobayashi and Shuji Kawai and Seiga Ito},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1995},
  volume={59 11},
  pages={
          2172-5
        }
}
A 0.4-kb ScaI-HpaI fragment, 199 bp upstream of the structural gene for alkaline endoglucanase, from the alkalophilic Bacillus sp. KSM-64, was found to be essential for the extracellular production of the enzyme by recombinant Bacillus subtilis cells. We constructed a new vector, pHSP64 (5.5 kb), using pHY300PLK and part of the 5' region of the endoglucanase that contained a possible promoter region. Using recombinant B. subtilis cells that carried this vector, very high production of two… 
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