Application of the salt stress to the protoplast cultures of the carrot (Daucus carota L.) and evaluation of the response of regenerants to soil salinity

@article{Kiekowska2019ApplicationOT,
  title={Application of the salt stress to the protoplast cultures of the carrot (Daucus carota L.) and evaluation of the response of regenerants to soil salinity},
  author={Agnieszka Kiełkowska and Ewa Grzebelus and Agnieszka Lis-Krzyścin and Katarzyna Ma{\'c}kowska},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2019},
  volume={137},
  pages={379-395}
}
AbstractThis is the first study to generate carrot plants for enhanced salinity tolerance using a single-cell in vitro system. Protoplasts of three carrot accessions were exposed to treatment by seven different concentrations of NaCl (10–400 mM). Salt concentrations higher than 50 mM decreased plating efficiency and those of 200–400 mM of NaCl completely arrested mitotic divisions of cultured cells. The protoplast-derived plants from the control and 50–100 mM NaCl treatment were subjected to an… CONTINUE READING

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