Application of the DiversiLab system for tracing the source of the mixed infections caused by Cryptococcus neoformans var. grubii from a patient with systemic lupus erythematosus

  title={Application of the DiversiLab system for tracing the source of the mixed infections caused by Cryptococcus neoformans var. grubii from a patient with systemic lupus erythematosus},
  author={Hongtao Dou and Yingchun Xu and Taisheng Li},
Two strains of Cryptococcus neoformans (PU 66 and PU112) were simultaneously isolated from a patient with systemic lupus erythematosus. We aimed to trace the source of the mixed infections. Multi‐locus sequence typing (MLST) and the DiversiLab system analyses were performed on the 2 clinical and 23 environmental C. neoformans from pigeon droppings, 11 from the home (H1) the patient visited, 12 from another home (H2) as control. All the strains were uniformly genotyped as C. neoformans var… 
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Molecular characterization of Cryptococcus neoformans isolated from the environment in Beijing, China
It is concluded that not only clinical but also environmental isolates of C. neoformans need to be investigated more deeply and more extensively.
Yeast identification by sequencing, biochemical kits, MALDI–TOF MS and rep-PCR DNA fingerprinting
To achieve higher accuracies of identification, the databases of commercial biochemical test kits, MALDI-TOF MS platforms, and DiversiLab automated rep-PCR DNA fingerprinting needs further enrichment, particularly for uncommonly encountered yeast species.


Microsatellite Typing of Clinical and Environmental Cryptococcus neoformans var. grubii Isolates from Cuba Shows Multiple Genetic Lineages
The genotypic segregation between clinical and environmental isolates from pigeon guano suggests additional source(s) of human cryptococcal infections, and the selected panel of microsatellite markers is an excellent tool to study the epidemiology of C. neoformans var.
Comparison of Genotypes Between Environmental and Clinical Isolates of Cryptococcus neoformans var. grubii Based on Microsatellite Patterns
No differences were found in antifungal susceptibility and capsule size between major environmental and clinical MLMT types, and two genotypes were recognized that distinguish majority of clinical and environmental strains.
Comparative Analysis of Environmental and Clinical Populations of Cryptococcus neoformans
The combined molecular data suggest that this environmental population of C. neoformans is predominantly clonal, although there was evidence for recent or past recombination.
Prevalence of the VNIc genotype of Cryptococcus neoformans in non-HIV-associated cryptococcosis in the Republic of Korea.
This study affirms VNIc to be the most prevalent genotype of C. neoformans isolated from non-HIV patients with cryptococcosis in the Republic of Korea andsimilar strains in China had serious underlying conditions, with cancer and liver disease being the most common.
Microsatellite typing and susceptibilities of serial Cryptococcus neoformans isolates from Cuban patients with recurrent cryptococcal meningitis
Recurrences of cryptococcal meningitis in 7 Cuban patients was not associated with development of drug resistance of the original strain but by an initial infection with different strains or a reinfection with a new strain.
Mixed Infections and In Vivo Evolution in the Human Fungal Pathogen Cryptococcus neoformans
It is demonstrated that mixed infections in humans are more common than previously thought, occurring in almost 20% of patients diagnosed with cryptococcosis, and the concept of one strain causing one infection does not hold true for C. neoformans and may apply to other environmentally acquired fungal pathogens.
Molecular Typing of IberoAmerican Cryptococcus neoformans Isolates
To acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase gene restriction fragment length polymorphsm analysis.
The isolation ofCryptococcus neoformans from pigeon droppings and serotyping of naturally and clinically sourced isolates in China
This is the first report on the isolation ofCryptococcus neoformans from pigeon droppings in China and their serotypes.C. neoformans colonies which produced brown colonies on caffeic acid-cornmeal
International Multicenter Evaluation of the DiversiLab Bacterial Typing System for Escherichia coli and Klebsiella spp
Despite local variations, the DL system has the potential to indicate the occurrence of clonal outbreaks in an international setting, provided there is strict adherence to standardized, reproducible DNA isolation methods and analysis protocols, all supported by a central database for profile comparisons.
Dual Infections with Pigmented and Albino Strains of Cryptococcus neoformans in Patients with or without Human Immunodeficiency Virus Infection in India
The identification of distinct cryptococcal isolates with either pigmented or white colony phenotypes on l-dihydroxyphenylalanine agar plates in three patients who presented with meningitis to the All India Institute of Medical Sciences in India suggests that mixed infections occur more commonly than is currently realized.