Application of the Apn2/MAT locus to improve the systematics of the Colletotrichum gloeosporioides complex: an example from coffee (Coffea spp.) hosts

@article{Silva2012ApplicationOT,
  title={Application of the Apn2/MAT locus to improve the systematics of the Colletotrichum gloeosporioides complex: an example from coffee (Coffea spp.) hosts},
  author={Diogo N. Silva and Pedro Talhinhas and Vitor V{\'a}rzea and Lei Cai and Oct{\'a}vio S. Paulo and Dora Batista},
  journal={Mycologia},
  year={2012},
  volume={104},
  pages={396 - 409}
}
To improve phylogenetic resolution of the Colletotrichum gloeosporioides species complex we developed and tested the performance of a new set of primers for the Apn2/MAT locus with a case study of 22 isolates. These were isolated mainly from coffee plants and represent six divergent and well characterized species within the C. gloeosporioides complex. Following previous studies on this locus, we have generated sequence data from an expanded region (> 4600 bp), revealing increased phylogenetic… 

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