Application of the Amplex red/horseradish peroxidase assay to measure hydrogen peroxide generation by recombinant microsomal enzymes.

@article{Mishin2010ApplicationOT,
  title={Application of the Amplex red/horseradish peroxidase assay to measure hydrogen peroxide generation by recombinant microsomal enzymes.},
  author={Vladimir M. Mishin and Joshua P. Gray and Diane E. Heck and Debra L. Laskin and Jeffrey D Laskin},
  journal={Free radical biology & medicine},
  year={2010},
  volume={48 11},
  pages={1485-91}
}
The formation of reactive oxygen species by the cytochrome P450 monooxygenase system is thought to be due to autoxidation of NADPH-cytochrome P450 reductase and the nonproductive decay of oxygen-bound cytochrome P450 intermediates. To characterize this process in recombinant microsomal enzymes, we used a highly sensitive hydrogen peroxide assay based on… CONTINUE READING