Application of spectrofluorometry to the prediction of PHB concentrations in a fed-batch process.

@article{Henneke2005ApplicationOS,
  title={Application of spectrofluorometry to the prediction of PHB concentrations in a fed-batch process.},
  author={Daniel Henneke and Andrea Hagedorn and Hector M. Budman and Raymond L. Legge},
  journal={Bioprocess and biosystems engineering},
  year={2005},
  volume={27 6},
  pages={359-364}
}
On-line estimation of biopolymer production during fermentation would be a useful adjunct to the development of strategies for process control and optimization. This study examined the applicability of spectrofluorometry, along with other on-line measurements, for the prediction of poly-beta-hydroxybutyric acid (PHB) concentrations in a high-cell density fed-batch fermentation of Ralstonia eutropha. Models previously used for modelling PHB evolution with time are not sufficiently accurate for… CONTINUE READING