Application of sonication to release DNA from Bacillus cereus for quantitative detection by real-time PCR.

@article{Fykse2003ApplicationOS,
  title={Application of sonication to release DNA from Bacillus cereus for quantitative detection by real-time PCR.},
  author={Else Marie Fykse and Jaran Strand Olsen and Gunnar Skogan},
  journal={Journal of microbiological methods},
  year={2003},
  volume={55 1},
  pages={1-10}
}
A rapid sonication method for lysis of Gram-positive bacteria was evaluated for use in combination with quantitative real-time polymerase chain reaction (PCR) analyses for detection. Other criteria used for evaluation of lysis were microscopic cell count, colony forming units (cfu), optical density at 600 nm and total yield of DNA measured by PicoGreen fluorescence. The aim of this study was complete disruption of cellular structures and release of DNA without the need for lysing reagents and… CONTINUE READING