Application of sequential integration for metabolic engineering of 1,2-propanediol production in yeast.

@article{Lee2006ApplicationOS,
  title={Application of sequential integration for metabolic engineering of 1,2-propanediol production in yeast.},
  author={Wonkyu Lee and Nancy A Dasilva},
  journal={Metabolic engineering},
  year={2006},
  volume={8 1},
  pages={58-65}
}
The yeast species Saccharomyces cerevisiae was engineered to produce 1,2-propanediol (1,2-PD) using the delta/UB sequential gene integration method. To study the effects of increased copy number, 2 genes (mgs and gldA from Escherichia coli) were sequentially integrated into the chromosomes of S. cerevisiae strains of opposite mating type. The resulting strains (containing 0-3 copies of either mgs or gldA) were mated to create all possible combinations of the 2 genes introduced for 1,2-PD… CONTINUE READING

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