Application of reversed phase high performance liquid chromatography for subproteomic analysis of cardiac muscle.

@article{Neverova2002ApplicationOR,
  title={Application of reversed phase high performance liquid chromatography for subproteomic analysis of cardiac muscle.},
  author={Irina Neverova and Jennifer E Van Eyk},
  journal={Proteomics},
  year={2002},
  volume={2 1},
  pages={22-31}
}
The application of protein separation methodologies, such as reversed phase chromatography, should allow differential separation of the proteome, or at least specific subproteomes, comparable to that achieved by two-dimensional electrophoresis (2-DE). A rapid sequential protein extraction method (termed "IN Sequence") was developed to isolate three distinct subproteomes of cardiac muscle. Two subproteomes, those enriched for the cytoplasmic or myofilament proteins, can be separated by either… CONTINUE READING

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