Corpus ID: 31590301

Application of redD, the transcriptional activator gene of the undecylprodigiosin biosynthetic pathway, as a reporter for transcriptional activity in Streptomyces coelicolor A3(2) and Streptomyces lividans.

@article{vanWezel2000ApplicationOR,
  title={Application of redD, the transcriptional activator gene of the undecylprodigiosin biosynthetic pathway, as a reporter for transcriptional activity in Streptomyces coelicolor A3(2) and Streptomyces lividans.},
  author={Gilles P. van Wezel and J. White and G Hoogvliet and M. J. Bibb},
  journal={Journal of molecular microbiology and biotechnology},
  year={2000},
  volume={2 4},
  pages={
          551-6
        }
}
redD encodes the transcriptional activator of the biosynthetic pathway for undecylprodigiosin, a red-pigmented, mycelium-bound antibiotic made by Streptomyces coelicolor A3(2) and Streptomyces lividans. [...] Key Result One plasmid, plJ2587, replicates autonomously in both Escherichia coli and streptomycetes, while the other, plJ2585, replicates in E. coli and can be transferred to streptomycetes by conjugation or transformation, whereupon it integrates stably at the chromosomal attachment site for the…Expand
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References

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bldA dependence of undecylprodigiosin production in Streptomyces coelicolor A3(2) involves a pathway-specific regulatory cascade
The production of the red-pigmented tripyrrole antibiotic undecylprodigiosin (Red) by Streptomyces coelicolor A3(2) depends on two pathway-specific regulatory genes, redD and redZ. RedD is homologousExpand
Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2)
TLDR
The putative redD polypeptide lacked any strong sequence similarities to other known proteins and was not involved in the regulation of undecylprodigiosin (Red) biosynthesis in Streptomyces coelicolor. Expand
afsR is a pleiotropic but conditionally required regulatory gene for antibiotic production in Streptomyces coelicolor A3(2)
TLDR
It is demonstrated that multiple copies of afsR can stimulate both Act and Red production and that, despite its homology, it cannot substitute for the pathway‐specific regulatory genes. Expand
xylE functions as an efficient reporter gene in Streptomyces spp.: use for the study of galP1, a catabolite-controlled promoter
TLDR
The properties of xylE as a reporter gene make it suitable not only for quantitatively monitoring expression of regulated promoters in Streptomyces spp. Expand
Green fluorescent protein as a reporter for spatial and temporal gene expression in Streptomyces coelicolor A 3 ( 2 )
2 Departamento de Microbiologı!a y Gene! tica, Instituto de Microbiologı!a Bioquı!mica, CSIC/Universidad de Salamanca, Edificio Departmental, Campus ‘Miguel de Unamuno’, 37007 Salamanca, Spain TheExpand
Green fluorescent protein as a reporter for spatial and temporal gene expression in Streptomyces coelicolor A3(2).
TLDR
The enhanced green fluorescent protein (EGFP) gene was inserted into plasmids that can replicate in Escherichia coli, greatly facilitating the construction of EGFP gene fusions and appeared to be confined to developing and mature spore chains, while transcription of redD occurred only in ageing substrate mycelium. Expand
The minimal replicon of a streptomycete plasmid produces an ultrahigh level of plasmid DNA.
TLDR
A functional map of Streptomyces coelicolor plasmid SCP2* was deduced from derivatives constructed by in vitro deletions and the tsr gene for thiostrepton resistance from StrePTomyces azureus were incorporated as selectable antibiotic resistance markers in streptomycetes. Expand
The mRNA for the 23S rRNA methylase encoded by the ermE gene of Saccharopolyspora erythraea is translated in the absence of a conventional ribosome‐binding site
TLDR
Site‐directed and deletion mutagenesis, combined with immunochemical analysis, demonstrated that the ermEp1 transcript is translated in the absence of a conventional ribosome‐binding site to give rise to the full‐length 23S rRNA methylase. Expand
Transcription analysis of the Streptomyces coelicolor A3(2) rrnA operon.
TLDR
Transcription from all of the rrnA promoters decreased during the transition from exponential to stationary phase, although transcription from P1 and P2 ceased several hours before that from P3 and P4. Expand
Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus.
TLDR
It is suggested that these sites represent a class of vegetatively expressed Streptomyces promoter that is utilised by a form of RNA polymerase holoenzyme that also recognizes typical promoters of other bacterial genera. Expand
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