Application of real-time PCR and RT-PCR assays for the detection and quantitation of VT 1 and VT 2 toxin genes in E. coli O157:H7.

@article{Fitzmaurice2004ApplicationOR,
  title={Application of real-time PCR and RT-PCR assays for the detection and quantitation of VT 1 and VT 2 toxin genes in E. coli O157:H7.},
  author={J. C. Fitzmaurice and Maura Glennon and Geraldine Duffy and James Sheridan and Cyril Carroll and Majella Maher},
  journal={Molecular and cellular probes},
  year={2004},
  volume={18 2},
  pages={
          123-32
        }
}
Real-time PCR assays, based on hybridisation probes and LightCycler technology, were developed for VT 1 and VT 2 genes and applied to the detection and quantitation of DNA and mRNA of Escherichia coli O157:H7. The qualitative consensus PCR assay for the detection of VT 1 and/or VT 2 genes had a detection limit of 100 fg of E. coli O157:H7 genomic DNA and did not detect DNA from 13 non-VTEC isolates. When E. coli O157:H7 was inoculated into minced beef, enriched and recovered by immunomagnetic… CONTINUE READING
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