Application of propidium monoazide quantitative PCR for selective detection of live Escherichia coli O157:H7 in vegetables after inactivation by essential oils.

@article{Elizaquvel2012ApplicationOP,
  title={Application of propidium monoazide quantitative PCR for selective detection of live Escherichia coli O157:H7 in vegetables after inactivation by essential oils.},
  author={Patricia Elizaqu{\'i}vel and Gl{\`o}ria S{\'a}nchez and Rosa Vercher Aznar},
  journal={International journal of food microbiology},
  year={2012},
  volume={159 2},
  pages={115-21}
}
The use of propidium monoazide (PMA) is enjoying increased popularity among researchers in different fields of microbiology. Its use in combination with real-time PCR (qPCR) represents one of the most successful approaches to detect viable cells. PMA-qPCR has successfully been used to evaluate the efficacy of various disinfection technologies in different microorganisms. Initially, in this study the effect of four essential oils (EOs), cumin, clove, oregano and cinnamon, was evaluated on… CONTINUE READING
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