Application of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) to identification of flatfish species.

@article{Cspedes1999ApplicationOP,
  title={Application of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) to identification of flatfish species.},
  author={{\'A}ngel Enrique C{\'e}spedes and Teresa Garcia and Erkuden P{\'e}rez Carrera and Isabel Gonz{\'a}lez and Alicia Calleja Fern{\'a}ndez and Pablo V. Hernandez and Rosario Mart{\'i}n},
  journal={Journal of AOAC International},
  year={1999},
  volume={82 4},
  pages={903-7}
}
A method of DNA analysis based on polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European plaice… CONTINUE READING

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