Application of nurse culture for plant regeneration from protoplasts of Lilium japonicum thunb

@article{Komai2007ApplicationON,
  title={Application of nurse culture for plant regeneration from protoplasts of Lilium japonicum thunb},
  author={Fuminori Komai and Hitomi Morohashi and Mitsugu Horita},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2007},
  volume={42},
  pages={252-255}
}
The regeneration of lily protoplasts isolated from suspension cells of Lilium japonicum was achieved by using the nurse culture method. The protoplasts divided only under the nurse culture application. The divided protoplasts grew into colonies and developed into visible calluses on a medium containing picloram. After the calluses were transferred to a hormone-free medium, plantlets formed from them. The highest frequency of plant regeneration was obtained on a medium containing 1 μM… CONTINUE READING

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