Application of native-state electrospray mass spectrometry to identify zinc-binding sites on engineered hemoglobin.

@article{Lippincott2000ApplicationON,
  title={Application of native-state electrospray mass spectrometry to identify zinc-binding sites on engineered hemoglobin.},
  author={Jon Lippincott and Timothy J. Fattor and Douglas D. Lemon and Izydor Apostol},
  journal={Analytical biochemistry},
  year={2000},
  volume={284 2},
  pages={247-55}
}
We report the utility of native-state mass spectrometry to detect zinc ion binding to the engineered hemoglobin rHb52. Various preparations of this recombinant hemoglobin had significantly different oxygen affinities. Detailed characterization of denatured globins did not show any difference between analyzed hemoglobin molecules. However, when solutions of… CONTINUE READING