Application of modern reversed-phase peptide retention prediction algorithms to the Houghten and DeGraw dataset: peptide helicity and its effect on prediction accuracy.

Abstract

Twenty five years ago Houghten and DeGraw published a groundbreaking study of reversed-phase (RP)-HPLC retention of 298 peptide analogs, including 260 peptides coding the positional substitution in a 13-mer molecule with all 20 naturally occurring amino acids [1]. The authors challenged the state-of-the-art assumption that peptide retention can be… (More)
DOI: 10.1016/j.chroma.2012.07.092

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