Application of microchip phosphate-affinity electrophoresis to measurement of protease activity in complex samples.

Abstract

We previously demonstrated microchip phosphate-affinity electrophoresis (μPAE) for activity measurement of kinases and phosphatases. In the current work, we extend the μPAE application to a protease: caspase-3 (CASP3). We designed a substrate peptide modified with both a fluorescent tag for label and a phosphate tag for separation. The CASP3 cleavage… (More)
DOI: 10.1016/j.ab.2012.09.016

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