Application of lentivirus in gene transduction of rat Glioma cells

@inproceedings{Weimin2007ApplicationOL,
  title={Application of lentivirus in gene transduction of rat Glioma cells},
  author={Wang Weimin},
  year={2007}
}
Objective To transfer herpes simplex viral thymidine kinase gene into rat glioma 9L cells using lentivirus.Methods The lentivirus-expressing plasmid pLenti6/V5-TK was constructed and identified by PCR,restriction enzymes and DNA sequence analysis.The identified pLenti6/V5-TK plasmid and the ViraPowerTM Packaging Mix were cotransfected into 293T cells to produce a replication-incompetent lentivirus.The cultured supernatant was collected 48 h later,and the lentivirus was added into rat glioma 9L… CONTINUE READING

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