Application of green fluorescent protein-labeled assay for the study of subcellular localization of Newcastle disease virus matrix protein.

@article{Duan2013ApplicationOG,
  title={Application of green fluorescent protein-labeled assay for the study of subcellular localization of Newcastle disease virus matrix protein.},
  author={Zhiqiang Duan and Qunhui Li and Liang He and Guo Zhao and Jian Chen and Shunling Hu and Xiufan Liu},
  journal={Journal of virological methods},
  year={2013},
  volume={194 1-2},
  pages={118-22}
}
Green fluorescent protein (GFP) used as a powerful marker of gene expression in vivo has so far been applied widely in studying the localizations and functions of protein in living cells. In this study, GFP-labeled assay was used to investigate the subcellular localization of matrix (M) protein of different virulence and genotype Newcastle disease virus (NDV) strains. The M protein of ten NDV strains fused with GFP (GFP-M) all showed nuclear-and-nucleolar localization throughout transfection… CONTINUE READING