Application of genome editing in aquatic farm animals: Atlantic salmon

@article{Wargelius2019ApplicationOG,
  title={Application of genome editing in aquatic farm animals: Atlantic salmon},
  author={Anna Wargelius},
  journal={Transgenic Research},
  year={2019},
  volume={28},
  pages={101 - 105}
}
  • A. Wargelius
  • Published 18 July 2019
  • Biology
  • Transgenic Research
Gene editing offers opportunities to solve fish farming sustainability issues that presently hampers expansion of the aquaculture industry. In for example Atlantic salmon farming, there are now two major bottlenecks limiting the expansion of the industry. One is the genetic impact of escaped farmed salmon on wild populations, which is considered the most long-term negative effect on the environment. Secondly and the utmost acute problem is the fish parasite salmon lice, which is currently… 

Genetic improvement technologies to support the sustainable growth of UK aquaculture

This review describes the state of the art in UK aquaculture breeding and stock supply, and how innovation in genetics technologies can help achieve the Scottish Government’s ambitious target of doubling its Aquaculture industry by 2030.

CRISPR/Cas Genome Editing—Can It Become a Game Changer in Future Fisheries Sector?

There is still a way to go before the CRISPR/Cas technology revolutionizes and becomes viable in commercial Aquaculture as the new breeding technology for aquaculture-important traits and species, and there are several technical challenges and regulatory and public issues concerning the applications.

A refinement to gene editing in Atlantic salmon using asymmetrical oligonucleotide donors

It is demonstrated that precise modification of the genome can be achieved in a single generation, allowing efficient introgression of favorable alleles and bypassing challenges associated with traditional selective breeding.

Genome editing in cultured fishes

Advances in knowledge of key molecular pathways and, in some cases, favorable alterations of phenotype have been achieved, and loss-of-function of myostatin led to increased muscle mass, greater weight, and greater fillet yield in genome-edited lines of red sea bream, tiger puffer, and Nile tilapia than in their unedited counterparts.

CRISPR-Cas Genome Editing Technique for Fish Disease Management: Current Study and Future Perspective

This study highlights the development of RNA-guided immunity to combat the Chilodonella protozoan group and nervous necrosis virus in marine finfish and highlights the immunological application of CRISPR-Cas against bacterial diseases in channel catfish and the white spot syndrome virus in shrimp.

Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors

It is suggested that HDR-induced SNR can be applied as a powerful NBT, allowing efficient introgression of favorable alleles and bypassing challenges associated with traditional selective breeding.

Genome editing on finfish: Current status and implications for sustainability

This review has retrieved the latest research on genome editing on aquacultured finfish species, exploring the technological progress and the scope.

Indel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9-mediated HDR in Atlantic salmon

Analysis of imperfect HDR variants suggest that repair occurs by synthesis-dependent strand annealing (SDSA), as it is shown for the first time in any species that indel location is dependent on template polarity.

The Ethics of Farm Animal Biotechnology from an Anthropological Perspective

These insights challenge the popular Neo-Darwinian account of unilateral adaptation only benefiting the more powerful party and support the hypothesis that humans do not just adapt, but actively shape the environment through cultural niche construction (CNC) that also involves care and protection for domesticated animals.

References

SHOWING 1-10 OF 14 REFERENCES

Genome editing in fishes and their applications.

  • Bo ZhuW. Ge
  • Biology
    General and comparative endocrinology
  • 2018

Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon

Investigating whether it is possible to produce germ cell-free salmon in F0 by using CRISPR-Cas9 to knock out dnd, a factor required for germ cell survival in vertebrates found it to be so, and revealing that sex differentiation of the somatic compartment does not depend on the presence of germ cells.

Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 Generation

This study shows for the first time successful use of the CRISPR/Cas9 technology in a marine cold water species and demonstrates that F0 fish can be used for functional studies in Atlantic salmon.

Editing of the Luteinizing Hormone Gene to Sterilize Channel Catfish, Ictalurus punctatus, Using a Modified Zinc Finger Nuclease Technology with Electroporation

This is the first sterilization achieved using ZFN technology in an aquaculture species and the first successful gene editing of channel catfish, and will help understand the roles of the LH gene, purposeful sterilization of teleost fishes, and is a step towards control of domestic, hybrid, exotic, invasive, and transgenic fishes.

Growth Enhancement in Transgenic Atlantic Salmon by the Use of an “All Fish” Chimeric Growth Hormone Gene Construct

An “all fish” growth hormone (GH) chimeric gene construct is developed by using an antifreeze protein gene (AFP) promoter from ocean pout linked to a chinook salmon GH cDNA clone to generate transgenic Atlantic salmon.

Targeted disruption of sp7 and myostatin with CRISPR-Cas9 results in severe bone defects and more muscular cells in common carp

The results demonstrate that both TALEN and CRISPR-Cas9 are highly efficient tools for modifying the common carp genome, and open avenues for facilitating common carp genetic studies and breeding.

Generation of albino medaka (Oryzias latipes) by CRISPR/Cas9

This method provides a detailed procedure to generate the genetic modification medaka by using an optimized CRISPR/Cas9 system, and the new albino medaka provides an important research platform to study the pigmentation.

Heritable Targeted Inactivation of Myostatin Gene in Yellow Catfish (Pelteobagrus fulvidraco) Using Engineered Zinc Finger Nucleases

To the best knowledge, this is the first endogenous gene knockout in aquaculture fish and will help in understanding the roles of mstn gene in fish.