Application of flow field flow fractionation-ICPMS for the study of uranium binding in bacterial cell suspensions.

@article{Jackson2005ApplicationOF,
  title={Application of flow field flow fractionation-ICPMS for the study of uranium binding in bacterial cell suspensions.},
  author={Brian P. Jackson and James F. Ranville and Andrew L. Neal},
  journal={Analytical chemistry},
  year={2005},
  volume={77 5},
  pages={1393-7}
}
Field flow fractionation (FFF) is a size-based separation technique applicable to biomolecules, colloids, and bacteria in solution. When interfaced with ICPMS on-line, elemental data can be collected concurrent with size distribution. We employed hyperlayer flow FFF (Fl FFF) methodology to separate cells of Shewanella oneidensis strain MR-1 from exopolymers present in washed cell suspensions. With a channel flow of 4 mL min-1 and a cross-flow of 0.4 mL min-1 cells eluted with a retention time… CONTINUE READING