Application of enzymes during bulb tissue extraction for detection of lily symptomless virus by ELISA in Lilium spp

@article{Beijersbergen2005ApplicationOE,
  title={Application of enzymes during bulb tissue extraction for detection of lily symptomless virus by ELISA in Lilium spp},
  author={J. C. M. Beijersbergen and C. Th.C. van der Hulst},
  journal={Netherlands Journal of Plant Pathology},
  year={2005},
  volume={86},
  pages={277-283}
}
Lily symptomless virus (LSV) was readily detected with the enzyme-linked immunosorbent assay (ELISA) in leaves ofLilium Mid-Century hybrids cvs Enchantment and Destiny andLilium speciosum cv. Brabander. In bulbs of cv. Enchantment LSV could be detected without any additional treatment of the extracts, but for reliable results in cv. Destiny it was necessary to pre-incubate the bulb extracts with cellulase or hemicellulase.SamenvattingIn de bladeren van secundair geïnfecteerde planten van de… 
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Purification of lily symptomless virus. Use and value of antisera against intact and pyrrolidine-degraded virus for testing lilies and tulips
TLDR
It was concluded that intact and degraded LSV have very few antigenic determinants in common or none at all and primary infections were detected more successfully in stored bulbs than in leaves taken from plants in the preceding growing season.
Purification of tulip breaking virus and production of anti-sera for use in ELISA
TLDR
Tulip breaking virus (TBV) was purified on a large scale by homogenization in 0.1 M tris buffer, clarification with Triton X-100, and differential centrifugation, and the purity of the product was verified by mechanical inoculation of test plants, serology, electron microscopy, spectrophotometry, and analytical ultracentrifugation.

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Purification of lily symptomless virus. Use and value of antisera against intact and pyrrolidine-degraded virus for testing lilies and tulips
TLDR
It was concluded that intact and degraded LSV have very few antigenic determinants in common or none at all and primary infections were detected more successfully in stored bulbs than in leaves taken from plants in the preceding growing season.
Brown ring formation and streak mottle, two distinct syndromes in lilies associated with complex infections of lily symptomless virus and tulip breaking virus
TLDR
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Enzyme-linked immunosorbent assay (ELISA) for the detection of potato viruses A and Y in potato leaves and sprouts
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The method enabled the highly sensitive detection of a number of morphologically different viruses in purified preparations and in unclarified extracts of herbaceous hosts and of infected crop plants.
Schadschnecken und ihre Bek/impfung. 467 pp., 128 figs, 51 tables and 12 pages
  • 1979
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