Application of denaturing high-performance liquid chromatography in microbial ecology: fermentor sludge, compost, and soil community profiling.

@article{Wagner2009ApplicationOD,
  title={Application of denaturing high-performance liquid chromatography in microbial ecology: fermentor sludge, compost, and soil community profiling.},
  author={Andreas Otto Wagner and Cornelia Malin and Paul Illmer},
  journal={Applied and environmental microbiology},
  year={2009},
  volume={75 4},
  pages={956-64}
}
Genetic fingerprinting methods, such as denaturing gradient gel electrophoresis (DGGE), are used in microbial ecology for the analysis of mixed microbial communities but are associated with various problems. In the present study we used a new alternative method: denaturing high-performance liquid chromatography (dHPLC). This method was previously shown to work with samples from water and gut flora but had not yet been applied to complex environmental samples. In contrast to other publications… CONTINUE READING

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