Application of cryotechniques in cartilage tissue preservation and immunoelectron microscopy: potentials and problems.

Abstract

Cryotechnical processing of cartilage has the potential to solve many of the tissue-specific problems associated with various routine chemical fixation protocols. This is particularly the case with respect to extracellular matrix architecture, the distortion or destruction of which (caused by extraction and/or precipitation of proteoglycan molecules) may be prevented. Adoption of such techniques also permits high-sensitivity immunoelectron-microscopy of the extracellular matrix space (carbohydrate epitopes). However, a number of difficulties still remain to be resolved, particularly that of matrix-cell interface separation occurring during freeze substitution and low temperature embedding. These problems are briefly addressed and possible solutions outlined.

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@article{Hunziker1993ApplicationOC, title={Application of cryotechniques in cartilage tissue preservation and immunoelectron microscopy: potentials and problems.}, author={Ernst Bruno Hunziker}, journal={Microscopy research and technique}, year={1993}, volume={24 6}, pages={457-64} }