Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species.

@article{Kusunoki1991ApplicationOC,
  title={Application of colorimetric microdilution plate hybridization for rapid genetic identification of 22 Mycobacterium species.},
  author={S. Kusunoki and T. Ezaki and M. Tamesada and Y. Hatanaka and K. Asano and Y. Hashimoto and E. Yabuuchi},
  journal={Journal of clinical microbiology},
  year={1991},
  volume={29 8},
  pages={
          1596-603
        }
}
Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization, hybridized DNAs were quantitatively detected with peroxidase-conjugated streptavidin and the substrate, tetramethylbenzidine. This method could differentiate among 20 of the 22… Expand
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