Application of an inducible system to engineer unmarked conditional mutants of essential genes of Pseudomonas aeruginosa.

@article{Morita2010ApplicationOA,
  title={Application of an inducible system to engineer unmarked conditional mutants of essential genes of Pseudomonas aeruginosa.},
  author={Yuji Morita and Shin-ichiro Narita and Junko Tomida and Hajime Tokuda and Yoshiaki Kawamura},
  journal={Journal of microbiological methods},
  year={2010},
  volume={82 3},
  pages={
          205-13
        }
}
The Phi CTX-based integration vector pYM101 harboring a tightly controlled modified phage T7 early gene promoter/LacI(q) repressor (T7/LacI) system was constructed for the generation of unmarked conditional mutants in Pseudomonas aeruginosa. Promoter activity of the T7/LacI system was demonstrated to be dependent on the presence of the inducer isopropyl -beta-D-1-thiogalactopyranoside (IPTG), as evaluated by measuring beta-galactosidase activity. In the absence of the inducer, the promoter was… CONTINUE READING
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