Application of an in vitro assay for serum thymidine kinase: Results on viral disease and malignancies in humans

  title={Application of an in vitro assay for serum thymidine kinase: Results on viral disease and malignancies in humans},
  author={J. Simon Gronowitz and Clas F. R. K{\"a}llander and Hans Diderholm and Hans Hagberg and U. Pettersson},
  journal={International Journal of Cancer},
An improved method for the detection of deoxythymidine kinase (TK) in human sera is reported. The method which utilizes 125I‐iododeoxyuridine (IdUrd) as a substrate was used to measure TK in sera from patients with different diseases. Sera collected during the acute stage of infectious mononucleosis were found to contain elevated levels of TK, in most cases 10–40 times the normal value. The serum TK activity disappeared gradually and reached a normal level within 4 weeks. Sera from patients… 

Changes of serum thymidine kinase in children with acute leukemia.

The study showed that the elevation of TK serum levels during follow-up was a helpful marker for the recognition of an early stage of relapse and in some cases occurred as early as one month before the appearance of clinical signs.

Diagnostic implications of the molecular forms and levels of serum thymidine kinase 1 in different canine malignancies

Overall, the results demonstrate that TK1 protein assays provide valuable diagnostic information in a variety of canine malignancies.

The Clinical Significance of Thymidine Kinase 1 Measurement in Serum of Breast Cancer Patients Using Anti-TK1 Antibody

In conclusion, the anti-TK1 antibody could be a good marker for monitoring the response of breast cancer patients to therapy.

Serum Thymidine Kinase (TK) Evaluation in HIV Infection

Serum TK was elevated from the beginning of the HIV infection, the seropositive stage, and more markedly afterwards during the course of the infection, with a close correlation with the stage.

Serum deoxythymidine kinase gives prognostic information in chronic lymphocytic leukemia

It is concluded that S‐TK could be used as a prognostic marker for the individual CLL patient and seems to be useful for longitudinal follow‐up studies of disease status, both in indolent disease and in progressive disease during treatment.

Serum Thymidine Kinase Activity as a Useful Marker for Bovine Leukosis

Results indicate that serum TK activity may be a marker for bovine leukosis, and the sensitivity and specificity of measuring serum Tk activity as a diagnostic tool for Bovine Leukosis was 95.0% and 95.9%, respectively.

Molecular properties of serum thymidine kinase 1 and use of the canine enzyme in disease monitoring

Investigating the molecular characteristics of canine thymidine kinase 1 and utilizing several anti-TK1 antibodies to a better understanding its role in disease prognostics, risk assessment, and monitoring of cancer development revealed that serum TK1 activity was elevated in 41% of bitches with pyometra, and thus TK 1 determinations may be clinically valuable also in some non-neoplastic diseases.

Serum thymidine kinase as a marker of disease activity in patients with multiple myeloma.

Detailed multiparameter longitudinal investigations of 5 patients and ongoing studies of 70 other patients suggest that STK is a more reliable marker of progressive disease than either SB2M, LI, M-protein or CD10 positive lymphocytes.

Serum deoxythymidine kinase as a progressive marker of hematological malignancy.

The results suggest that high levels of s-dTK in hematological malignancy may reflect the extent of disease and that this activity may be derived from proliferating leukemic cells.



Rapid diagnosis of varicella-zoster virus infection by detection of viral deoxythymidine kinase in serum and vesicle fluid

A sensitive enzyme assay utilizing [125I]iododeoxyuridine as the substrate and CTP as the phosphate donor in combination with isozyme-specific antisera was used for direct detection and typing of

The use of serum deoxythymidine kinase as a prognostic marker, and in the monitoring of patients with non-Hodgkin's lymphoma.

It is concluded that s-dTk could be used both as a prognostic marker and to monitor the effect of therapy in NHL patients.

Optimized assay for thymidine kinase and its application to the detection of antibodies against herpes simplex virus type 1- and 2-induced thymidine kinase

An easily detectable blocking activity, which was shown to be mainly confined to the immunoglobulin G antibody class, was found in most human sera which were positive for complement-fixing antibody against herpes simplex virus.

Deoxycytidine and deoxythymidine kinase activities in plasma of mice and patients with neoplastic disease.

In C57BL x DBA/2 F1 (hereafter called BD2F1) mice inoculated with P815 neoplasms and in AKR mice with spontaneously developing leukemia, significant amounts of plasma deoxycytidine and thymidine

Enzyme patterns in a group of transplantable mouse hepatomas of different growth rates.

Deoxythymidine kinase and aspartate transcarbamylase activities correlated to some extent with the growth rate of these hepatomas, while threonine-serine dehydrase, histidase, and carbamylphosphate synthetase activities were almost undetectable in all the hepatomas.

Transformation of foetal human leukocytes in vitro by filtrates of a human leukaemic cell line containing herpes‐like virus

Preliminary studies found that the transforming factor was found to be sensitive to heat and ether, was sedimented at 70,000 × g and retained by a filter of APD 50 mμ; these results are compatible with the herpes‐like virus of the QIMR‐WIL cells being the transforming factors.

Rapid typing of herpes simplex virus based on immunological specificity of viral thymidine kinase and typing according to sensitivity to iododeoxyuridine

Two methods for typing of herpes simplex virus (HSV) are described and the detection and typing of HSV deoxythymidine kinase present in vesicle fluids is reported.

Prognostic relevance of thymidine kinase isozymes in adult non-Hodgkin's lymphoma.

It is suggested that peripheral blood TK1 isozyme is a useful independent biochemical marker for a subgroup of NHL who respond poorly to current therapy and thus require new therapeutic approaches.

Thymidine kinase in rat liver during development.

The decline in enzyme activity during the neonatal period was correlated with a shift in the enzyme properties from the foetal to the adult type, and may reflect the decrease in the proportion of haemopoietic tissue in the liver.