Application of an improved proteomics method, fluorogenic derivatization-liquid chromatography-tandem mass spectrometry, to differential analysis of proteins in small regions of mouse brain.

@article{Asamoto2008ApplicationOA,
  title={Application of an improved proteomics method, fluorogenic derivatization-liquid chromatography-tandem mass spectrometry, to differential analysis of proteins in small regions of mouse brain.},
  author={Hiromichi Asamoto and Tomoko Ichibangase and Kazuo Uchikura and Kazuhiro Imai},
  journal={Journal of chromatography. A},
  year={2008},
  volume={1208 1-2},
  pages={147-55}
}
To identify age-related proteins in small regions of mouse brain, we improved a proteomics approach, fluorogenic derivatization-liquid chromatography-tandem mass spectrometry (FD-LC-MS/MS), and applied the method to the differential proteome analysis of aging in cerebral cortex, hippocampus and brainstem. The method showed good accuracy with RSDs <10% for between-day protein peak heights, and much better sensitivity for the detection of proteins compared to other proteomics approaches. The… CONTINUE READING