Application of an NaOH maceration method to a scanning electron microscopic observation of Ito cells in the rat liver.

@article{TakahashiIwanaga1986ApplicationOA,
  title={Application of an NaOH maceration method to a scanning electron microscopic observation of Ito cells in the rat liver.},
  author={Hiromi Takahashi-Iwanaga and Tsuneo Fujita},
  journal={Archivum histologicum Japonicum = Nihon soshikigaku kiroku},
  year={1986},
  volume={49 3},
  pages={
          349-57
        }
}
A three-dimensional observation of the Ito cells in the rat liver was made by scanning electron microscopy (SEM) with an NaOH maceration method. The treatment with NaOH facilitated the intercellular separation of the specimen and completely removed reticular fibers around the sinusoid. In this way, the entire shapes of the Ito cells located in the perisinusoidal spaces or between the hepatocytes, were exposed under the SEM. The Ito cells were disposed at regular intervals of 30-55 microns all… CONTINUE READING

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